The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation.
نویسندگان
چکیده
Single-celled cotton fiber (Gossypium hirsutum) provides a unique experimental system to study cell elongation. To investigate the role of the actin cytoskeleton during fiber development, 15 G. hirsutum ACTIN (GhACT) cDNA clones were characterized. RNA gel blot and real-time RT-PCR analysis revealed that GhACT genes are differentially expressed in different tissues and can be classified into four groups. One group, represented by GhACT1, is expressed predominantly in fiber cells and was studied in detail. A 0.8-kb GhACT1 promoter sufficient to confirm its fiber-specific expression was identified. RNA interference of GhACT1 caused significant reduction of its mRNA and protein levels and disrupted the actin cytoskeleton network in fibers. No defined actin network was observed in these fibers and, consequently, fiber elongation was inhibited. Our results suggested that GhACT1 plays an important role in fiber elongation but not fiber initiation.
منابع مشابه
Study on the Damaging of Dyed Cotton Fibers with Direct Dye in Spinning Processes and its Effect on the Properties of Cotton Mélange Yarn
The influence of dyeing and spinning on the characteristics of cotton fibers and its impact on the properties of cotton mélange yarn has been investigated. Grey cotton fibers with mean fiber length and fineness of 29 mm and 4.2 micronair were pre-treated and dyed. Three ring yarns were spun from 100 % grey cotton (R.R.Y), 50-50 % blend of dyed and grey cotton (M.R.Y) and 100 % dyed cotton fiber...
متن کاملCotton annexin proteins participate in the establishment of fiber cell elongation scaffold
Cotton plant is one of the most important economic crops in the world which supplies natural fiber for textile industry. The crucial traits of cotton fiber quality are fiber length and strength, which are mostly determined by the fiber elongation stage. Annexins are assumed to be involved in regulating fiber elongation, but direct evidences remain elusive. Recently, we have investigated the act...
متن کاملطراحی، ساخت و تأیید مولکولی سازۀ جفتی بیان کنندۀ ژن MaSp1 و بیان گذرای آن در الیاف پنبه
Cotton (Gossypium hirsutum L.) is an important crop in the world and increasing its fiber quality is very cr-ucial for textile industries. Spider silk is the strongest and most elastic fiber ever known in the nature. Cotton is one of the main crops in the world and increasing its fiber quality is very important for textile industries. In this regard, a synt-hetic construct has been designe...
متن کاملIdentification and Profiling of microRNAs Expressed in Elongating Cotton Fibers Using Small RNA Deep Sequencing
Plant microRNAs (miRNAs) have been shown to play essential roles in the regulation of gene expression. In this study, small RNA deep sequencing was applied to explore novel miRNAs expressed in elongating cotton fibers. A total of 46 novel and 96 known miRNAs, primarily derived from the corresponding specific loci in genome of Gossypium arboreum, were identified. 64 miRNAs were shown to be diffe...
متن کاملProteomic Identification of Differentially Expressed Proteins in the <italic>Ligon lintless</italic> Mutant of Upland Cotton (<italic>Gossypium hirsutum</italic> L.)
Cotton fiber is an ideal model for studying plant cell elongation. To date, the underlying mechanisms controlling fiber elongation remain unclear due to their high complexity. In this study, a comparative proteomic analysis between a short-lint fiber mutant (Ligon lintless, Li1) and its wild-type was performed to identify fiber elongation-related proteins. By 2-DE combined with local EST databa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Plant cell
دوره 17 3 شماره
صفحات -
تاریخ انتشار 2005